Column-Free CD14+ Monocyte Isolation using 50nm Superparamagentic Beads on MARS® Bar

Professional antigen-presenting cells, dendritic cells are essential for controlling immune responses. Numerous clinical trials have employed dendritic cells as both medications and vaccines for the treatment of cancer. As crucial innate immune cells, macrophages can mediate the phagocytosis of hazardous cells or materials and take part in efficient tissue regeneration when they become activated. Therefore, cell-based therapeutics using ex vivo-generated macrophages have been tested in clinical settings. Peripheral blood monocytes can be used to produce dendritic cells and macrophages. In order to facilitate reliable, repeatable, and streamlined monocyte isolation processes, new products and technologies are required.
50 nm superparamagnetic particles have a larger surface area than conventional micron-sized beads, which are known to have low efficiency and require a de-beads procedure before being used in downstream applications. Clinical applications have made use of 50nm magnetic beads that are commercially accessible. For effective separation, though, columns must be utilized with the 50 nm magnetic beads that are currently on the market. Here, we demonstrate the isolation of CD14+ monocytes on a closed, automated system MARS® Bar utilizing Applied Cells IngenuityTM 50 nm beads and our unique column-free technique.