The spectral approach shows promise in adding flexibility to panel design and improving the measurement of fluorescent signal. However, few comparisons between conventional and spectral systems have been reported to date. Here we present our findings comparing conventional and spectral approaches to cytometry—including comparisons of compensation and unmixing—and evaluate the use of spectral cytometry for immune profiling in viral diseases.
Learning Objectives
- Gain an understanding of the essential differences between conventional and spectral approaches to cytometry.
- Appreciate the differences between compensation and spectral unmixing.
- Consider applications for spectral cytometry in the context of immunological studies.
Thomas Ashhurst, PhD, Immunologist and High-Dimensional Cytometry Specialist, Sydney Cytometry Facility
April 21, 2021, 10:00 PM (CEST)
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