Calcium signaling is essential for cellular communication, orchestrating a myriad of physiological processes. As the quest for a more profound understanding of cellular dynamics intensifies, calcium flux analysis emerges as a vital tool for deciphering cell signaling and cellular metabolism. While receptor-stimulated calcium influx serves as a routine metric for cell signaling, conventional techniques often fall short in terms of sensitivity and specificity. Given the diverse array of available calcium indicator dyes, the careful selection and optimization of reagents is imperative for precise calcium flux assessments. The research described in this webinar harnesses the innovative capabilities of FSP technology to perform advanced kinetic analysis, thereby, facilitating real-time monitoring of calcium influx in primary murine T-cells.
Key topics discussed in this webinar will include:
- Calcium signaling in cellular processes
- The selection and optimization of reagents in calcium flux assays with a focus on Indo-1 AM
- Ways to refine calcium flux assay methodologies
- The integration of surface markers in multiplexing with Indo-1 AM
- Facilitating kinetic analysis and monitoring in calcium flux studies
Who should attend?
- Those with an interest in basic and/or clinical studies of cellular calcium flux and signaling
- Those with a general interest in the development and optimization of flow cytometry assays
- Anyone intereste in the fundamentals of flow cytometry
Loni Perrenoud,Technical Application Specialist, Cytek Biosciences
Date: January 18, 2024
Time: 5:00 PM (CET)
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