Bioluminescence imaging relies on precise D-luciferin administration, as dosing variability impacts signal intensity and data consistency. Standardizing substrate delivery is essential for reproducible and high-quality in vivo imaging results.
Mice were imaged under anaesthesia (2.5% isoflurane in oxygen), daily until the bioluminescence signal disappeared. Imaging was performed in an Ami-HTX Small Animal Imager and analysed with Aura Imaging Software, from Spectral Instruments Imaging.
The anesthetized mouse was placed on a customized stage for tumor immobilization and colon tumor was exposed to intravital microscopy (IVM-MS2, IVIM Technology, Korea) equipped with 25× water immersion objective.
The aim of this guideline is to provide recommendations for the implementation of an effective and efficient quality control (QC) program for SPECT and PET systems in a preclinical imaging lab. Published online: 15 July 2024
Don't miss the Lunch Symposium on March 12th, at 12:45 PM, in Room LS 01, during EMIM 2025!
Our colleague Igor Valerian is available for meetings at Bruker's booth 001 at EMIM on March 12th, in Bilbao, Spain.
March 6, 2025
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