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Automated NGS library preparation using the Biometra TRobot II
A key success factor in automated library preparation is the performance of the integrated thermal cycler. Many NGS workflows depend on precise and reproducible temperature control during multiple sensitive enzymatic reaction steps such as fragmentation, end repair, dA-tailing, adapter ligation, reverse transcription, and PCR enrichment.
The Biometra TRobot II was developed specifically for integration into automated workflows and supports reliable 24/7 operation, making it well suited for high-throughput NGS approaches. The Biometra TRobot II combines a compact footprint, robotic access from three sides, and a plate lifting function that supports seamless unloading of plates. These features help minimize integration complexity on liquid handling platforms while improving robustness in automated operation. Furthermore, the instrument provides outstanding heating and cooling rates and excellent temperature homogeneity across the sample block, both of which are critical for reproducible enzymatic reactions and consistent amplification.
Related technologies: High-performance standard PCR and qPCR thermal cyclers
