Combining GrowDex® and spheroONE for automated and highly reproducible 3D cell culture workflows

This application note demonstrates the compatibility of spheroONE and GrowDex® to automate
3D cell culture workflows. spheroONE is a nanoliter dispenser enabling sorting and isolation of
spheroids and organoids. GrowDex® is a plant-derived nanofibrillar cellulose (NFC) hydrogel that
provides an effective 3D matrix for culturing various cell types. In this work, it was demonstrated
that bulk samples of HepG2 spheroids could be seamlessly grown from single HepG2 cell seeded
in this nanofibrillar cellulose matrix. Subsequently, spheroONE could be used to automate
dispensing of GrowDex® into microplates prior to sorting, isolation and embedding of single
HepG2 spheroids per well to prepare highly reproducible assay-ready microplates for subsequent
screening applications.

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