Get your cells ready for NGS with tiples nanodispenser MANTIS

Single-cell omics deliver precise information about individual cells, including transcription levels and the sequences of highly variable proteins. RNase H-dependent PCR-enabled T cell receptor sequencing (rhTCRseq) facilitates the efficient and highly specific examination of TCR domain diversity to characterize the immune response. By miniaturizing the assay, it becomes possible to conduct analysis at the single-cell level while maintaining sensitivity and data quality. A critical aspect of miniaturization is the need for low-volume liquid handling, which is not efficiently achievable manually and requires automation. This application note discusses the use of the MANTIS® liquid dispenser to create rhTCRseq cDNA libraries that are of quality comparable to those generated from bulk RNA.

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