More protein in less time: Automated native membrane protein purification with CyBio FeliX

Traditional solvent‑based methods of membrane protein purification have hindered research and development making protocols complicated, experimental conditions harsh, results unreliable and success rate of projects low. CyBio FeliX automation of Cube Biotech’s NativeMPTM copolymers and PlateX MPTM protocol revolutionize the study of cell membrane proteins making purification fast, consistent and efficient.

The automated protocol itself is full‑walk away and lasts a few hours compared to the days required by traditional methods. NativeMPTM avoids the use of detergents enabling the purification of stable proteins in their native tridimensional structures. Thus, compared to the use of solvent‑based methods, copolymer‑based purification gives samples closer to the environment in vivo that can give more biological significant results in downstream analysis.

The efficiency of the CyBio FeliX automation resulted in dramatically increased yields in purified protein, compared with manual methods. Moreover, stability studies showed that nanodisk‑embedded proteins are stable for longer and in harsher conditions. The automated PlateX MPTM method optimizes the screening of the most suitable copolymer for each protein and intended downstream experiment. This is particularly important in case of sensitive and costly experiments, such as Cryo‑EM for which the selection of the best copolymer backbone and protein physico‑chemical properties are essential.

Most importantly, this application note show how CyBio FeliX and PlateX MPTM can streamline the achievement of biologically significant results and projects success by transforming purification into a routine, scalable process and providing high yield of proteins which can be directly used for downstream analysis where good quality samples are critical.

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